Life Sciences CN on Swabian Instruments

Fluorescence Lifetime Flow Cytometry (FLFC)

Wed, 25 Feb 2026 00:00:00 +0000

Introduction to Fluorescence Lifetime Flow Cytometry

Flow cytometry is a sheath-flow-based technique that uses laser excitation and fluorescence to evaluate the physical and chemical characteristics of cells or other suspended particles in a fluid stream ¹. As these particles pass through the laser beam, the scattered light and fluorescence from labeled antibodies or markers can be used to obtain information on size, granularity, and molecular expression at the single-cell level, at rates exceeding tens of thousands of cells per second ². Flow cytometry is widely used across biomedical research and clinical diagnostics, including oncology, immunology, stem cell studies, microbiology, and synthetic biology. It offers high-throughput quantitative analysis of heterogeneous cell populations, which is crucial in a variety of applications such as cancer phenotyping, vaccine development, and rapid diagnosis of blood cancers or infectious diseases ³ ⁴.

Dynamic Light Scattering (DLS) Particle Size Analysis

Mon, 08 Sep 2025 00:00:00 +0000

Next-generation multi-angle DLS turnkey design with built-in consistency checks, raw photon access, and intelligent spike filtering for fast, reliable particle sizing.

Fluorescence Lifetime Imaging (FLIM)

Tue, 01 Jan 2019 00:00:00 +0000

Discover fluorescence lifetime imaging (FLIM), a powerful imaging technique for mapping fluorescence lifetimes with picosecond precision. Learn how Swabian Instruments’ Time Taggers enable high-resolution FLIM measurements using advanced timing electronics, supporting detectors like PMTs, SPADs, and SNSPDs for cutting-edge research applications

Time-Resolved Photoluminescence (TRPL)

Wed, 15 Oct 2025 00:00:00 +0000

Introduction

Introduction to Time-Resolved Photoluminescence (TRPL)

Time-resolved photoluminescence (TRPL) is a powerful technique that measures the photoluminescence decay (emission lifetime) of materials after pulsed excitation, thereby probing their optical and electronic properties. By tracking how excited states relax back to the ground state, TRPL distinguishes between radiative and non-radiative pathways, yielding lifetimes that report on charge-carrier dynamics, trap/defect activity, and other loss mechanisms. Compared with steady-state photoluminescence (PL), which focuses on spectral intensity, TRPL resolves the temporal decay of the luminescence signal, enabling quantitative extraction of recombination rates, multi-exponential or distributed lifetimes, and diffusion- or transfer-limited behavior. These parameters are broadly useful for screening and optimizing photonic materials, such as semiconductors, perovskites, polymers, and quantum-emitter platforms, and for interpreting contrast in bioimaging and other applications.